Lot # Check on the product label
Clone # N14
Human, mouse, rat
Product Form Liquid
Purification Protein A or Protein G purified
A synthetic peptide (conjugated with KLH) corresponding to the C-terminal of mouse 14-3-3 beta / alpha.
Western Blot (1:1000-2000)
Immunocytochemistry, ICC (1:100)
Immunohistochemistry, IHC-P (1:200)
Other applications have not been tested.
The optimal dilutions should be determined by end user.
In TBS (pH7.4), 0.5% BSA, 25% Glycerol and 0.05% Sodium Azide.
Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).
Avoid repeated freeze and thaw cycles.
14-3-3 proteins are a family of conserved regulatory molecules expressed in all eukaryotic cells. They have the ability to bind a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors. More than 100 signaling proteins have been reported as 14-3-3 ligands, 14-3-3 b/a is one of them. They play important roles in a wide range of vital regulatory processes, such as mitogenic signal transduction, apoptotic cell death, and cell cycle control. Elevated amounts of 14-3-3 proteins are found in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease.
1. Takahashi H, Iwata T, Kitagawa Y et al. (November 1999). "Increased levels of epsilon and gamma isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease". Clin. Diagn. Lab. Immunol. 6 (6): 983–5