Overview

Catalog# BMA1005

Lot # Check on the product label

Size 100μg

Isotype IgG1

Clone # S5-71

Host Mouse

Reactivity
Human, mouse, rat

Product Form Liquid

Purification Protein A or Protein G purified

Immunogen
A synthetic peptide (conjugated with KLH) corresponding to the C-terminal of APP.

Recommend Application
Western Blot (1:500-1000)
Other applications have not been tested.
 The optimal dilutions should be determined       by end user.

Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction
Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).
Avoid repeated freeze and thaw cycles.

Background
Amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. It is encoded by the APP gene which contains 19 exons and spans more than 170Kb^[1], APP has several isoforms, with certain isoforms preferentially expressed in neurons; changes in the neuronal ratio of these isoforms have been associated with Alzheimer's disease. ^[2] Several mutations outside the Aβ region associated with familial Alzheimer's have been found to dramatically increase production of Aβ.^[3]

Reference
1.    Yoshikai, S., Sasaki, H., Doh-ura, K., Furuya, H., Sakaki, Y. Genomic organization of the human amyloid beta-protein precursor gene. Gene 87: 257-263, 1990. Note: Erratum: Gene 102: 291 only, 1991.
2.    Matsui T, Ingelsson M, Fukumoto H, Ramasamy K, Kowa H, Frosch MP, Irizarry MC, Hyman BT (August 2007). "Expression of APP pathway mRNAs and proteins in Alzheimer's disease". Brain Res. 1161: 116–23.
3.    Citron M, Oltersdorf T, Haass C, McConlogue L, Hung AY, Seubert P, Vigo-Pelfrey C, Lieberburg I, Selkoe DJ (December 1992). "Mutation of the beta-amyloid precursor protein in familial Alzheimer's disease increases beta-protein production". Nature 360 (6405): 672–4.

Gene Information

OMIM:104760
Swiss Port:P05067
Gene ID:351