Overview

Catalog# BRP1099-1

Lot # Check on the product label

Size 100 μg

Description
Recombinant mature human AB1-42 protein.

Synonyms Beta Amyloid(1-42), beta Amyloid 1-42, beta-Amyloid 1-42, Amyloid 1-42, A4, ABETA, ABPP, Alzheimers Disease Amyloid Protein, Amyloid B, Amyloid Beta A4 Protein Precursor, Amyloid Beta, Amyloid of Aging and Alzheimer Disease, B Amyloid, Beta APP, Cerebral Vascular Amyloid Peptide, Beta-amyloid protein 42

Source  E.Coli BL21

Purity >85% by SDS-PAGE

Formulation  Liquid

Application
ELISA
Western blot, WB
Other applications have not been tested.
The optimal dilutions should be determined by end user.

Storage buffer
Each vial contains 0.01M PBS, pH 7.5.

Storage & Expiration
Ship at 4℃. Upon receipt, aliquot and store at -20℃ for long term.
Avoid repeated freeze and thaw cycles.

Background

Amyloid beta (Aβ or Abeta) denotes peptides of 36–43 amino acids that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. Oligomeric amyloid-β 1-42 (Aβ-42) peptides are considered to be the most toxic species connected to the occurrence of Alzheimer's disease. Unstructured oligomeric Aβ-42 interacted with membrane bilayers of giant unilamellar vesicles (GUV) and neuronal model cells, facilitated cellular uptake of Aβ-42, and inhibition of mitochondrial activity. Elevated concentrations of glucose within the range observed in diabetic individuals (10 mM) facilitate the formation of membrane-active Aβ-42 oligomers.

Reference
1. Kedia N, Almisry M, Bieschke J. Glucose directs amyloid-beta into membrane-active oligomers. Phys Chem Chem Phys. 2017 Jul 21;19(27):18036-18046.

Gene Information