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Anti-E2b Tag antibody
Catalog# BTL1004
Lot # Check on the product label
Size 100 μg
Isotype IgG1
Host Mouse
Reactivity
All
Specificity
Recognize C-terminal, internal and N-terminal E2b tagged proteins.
Product Form Liquid (1mg/ml)
Immunogen
A synthetic peptide GVSSTSSDFRDR conjugated to KLH.
Recommend Application
Western Blot (1:1000-10000)
Other applications have not been tested.
The optimal dilutions should be determined by end user.
Storage Buffer
In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.
Storage Instruction
Store at -20°C for 1 year. Or aliquot and store at -80°C for long term.
Avoid repeated freeze and thaw cycles.
Lot # Check on the product label
Size 100 μg
Isotype IgG1
Host Mouse
Reactivity
All
Specificity
Recognize C-terminal, internal and N-terminal E2b tagged proteins.
Product Form Liquid (1mg/ml)
Immunogen
A synthetic peptide GVSSTSSDFRDR conjugated to KLH.
Recommend Application
Western Blot (1:1000-10000)
Other applications have not been tested.
The optimal dilutions should be determined by end user.
Storage Buffer
In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.
Storage Instruction
Store at -20°C for 1 year. Or aliquot and store at -80°C for long term.
Avoid repeated freeze and thaw cycles.
Background
E2 Tags are 10-amino acids (epitope E2a: SSTSSDFRDR)- and 12 amino acids (epitope E2b: GVSSTSSDFRDR)-long peptides derived from the E2 protein of bovine papillomavirus type 1. The modules for C-terminal tagging with E2a and E2b epitopes were constructed by the modification of the pYM-series plasmid. The N-terminal E2a and E2b tagging modules were based on pOM-series plasmid.
E2 Tags are 10-amino acids (epitope E2a: SSTSSDFRDR)- and 12 amino acids (epitope E2b: GVSSTSSDFRDR)-long peptides derived from the E2 protein of bovine papillomavirus type 1. The modules for C-terminal tagging with E2a and E2b epitopes were constructed by the modification of the pYM-series plasmid. The N-terminal E2a and E2b tagging modules were based on pOM-series plasmid.
Reference
1. Tiina Tamm. Plasmids with E2 epitope tags: tagging modules for N- and C-terminal PCR-based gene targeting in both budding and fission yeast, and inducible expression vectors for fission yeast. Yeast Volume 26, Issue 1, pages 55–66, January 2009.
1. Tiina Tamm. Plasmids with E2 epitope tags: tagging modules for N- and C-terminal PCR-based gene targeting in both budding and fission yeast, and inducible expression vectors for fission yeast. Yeast Volume 26, Issue 1, pages 55–66, January 2009.
