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Propidium Iodide Solution

Catalog #BAD1009
Size100 T
In StockYes
Datasheet MSDS
Catalog# BAD1009

Size: 100 T

Lot # Check on the product label

Propidium iodide (or PI) is an intercalating agent and a fluorescent molecule with a molecular mass of 668.4 Da that can be used to stain cells. When excited with 488 nm wavelength light, it fluoresces red. PI binds to both DNA and RNA, so the latter must be removed by digestion with ribonucleases. The content of DNA as determined by flow cytometry, can reveal useful information about the cell cycle and the proteins involved in the regulation of the cell cycle. This reagent may be used to analyze cell cycle by flow cytometry in addition to use with antibodies for examining the expression of proteins during the cell cycle.

Storage Buffer
In PBS (pH7.2), 0.09% NaN3

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Application
Flow cytometry
Propidium Iodide Solution can be used in evaluation of apoptosis, cell viability and cell cycle analysis by flow cytometry.      

This reagent is used to analyze cell cycle by flow cytometry. It needs not dilution. Use 5 μL /test and incubate for 5 minutes at room temperature before analysis.

This solution contains hazardous material, please handle with care.