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7-AAD Viability Staining Buffer

Catalog # BAD1010
Size100 T
In stockYes
Datasheet MSDS
Catalog # BAD1010

Size: 100 T

Lot # Check on the product label

7-Aminoactinomycin D (7-AAD) is a fluorescent chemical compound with a strong affinity for DNA. It is used as a fluorescent marker for DNA in fluorescence microscopy and flow cytometry. It intercalates in double-stranded DNA, with a high affinity for GC-rich regions, making it useful for chromosome banding studies. With an absorption maximum at 546 nm, 7-AAD is efficiently excited using a 543 nm helium-neon laser; it can also be excited with somewhat lower efficiency using a 488 nm or 514 nm argon laser lines.

Storage Buffer
In PBS (pH7.2), 0.09% NaN3

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Application
Flow cytometry      

This reagent is used to analyze cell cycle by flow cytometry. It need not dilution. Use 5 μL /test and incubate for 5 minutes at room temperature before analysis.