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Activin A Rat ELISA Kit

Catalog #BEK1004
Size96 T
In stockYes
Datasheet MSDS

Activin A, the homodimer of the beta-A subunit, is a cytokine member of the transforming growth factor-beta superfamily. It is produced in the gonads, pituitary gland, placenta, and other organs, and is expressed locally by the mesenchymal component of the hemopoietic microenvironment. It had a mitogenic effect on mouse osteoblastic cells and suppressed their alkaline phosphatase activity. It also has an important role in the inflammatory response and that FST may have significant therapeutic potential to reduce the severity of inflammatory diseases.

Principle of the Assay  
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Activin A polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Activin A polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were
used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Activin A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Activin A can be calculated.

Range: 62.5 pg/ml-4000 pg/ml

Sensitivity < 12 pg/ml

Storage and Expiration: Store at 2-8℃ for 4 months, or at -20℃ for 8 months.

Application: For quantitative detection of Activin A in serum, body fluids, tissue lysates or cell culture supernates.

Kit components
1.     One 96-well plate pre-coated with anti-Rat Activin A antibody
2.     Lyophilized Activin A standards: 2 tubes (10 ng / tube)                    
3.     Sample / Standard diluent buffer: 30 ml
4.     Biotin conjugated anti-Rat ACTIVIN A antibody (Concentrated): 130 μl. Dilution: 1:100
5.     Antibody diluent buffer: 12 ml
6.     Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130 μl. Dilution: 1:100
7.     ABC diluent buffer: 12 ml
8.     TMB substrate: 10 ml
9.     Stop solution: 10 ml
10.   Wash buffer (25X): 30 ml
Note: Reconstitute standards and test samples with Kit Component 3.

Gene Informaiton
OMIM: 147290
Swiss Port: P18331
Gene ID: 29200

Other Information
Please check datasheet for protocol.