Bone morphogenetic protein 2 (BMP-2) belongs to the TGF-β superfamily of proteins. Like other family members, it plays an important role in the development of bone and cartilage. It has been demonstrated to potently induce osteoblast differentiation in a variety of cell types.BMP-2 has an ability to induce the formation of ectopic cartilage when implanted subcutaneously.
Principle of the Assay
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- BMP-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- BMP-2 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BMP-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BMP-2 can be calculated.
Range: 62.5 pg/ml-4000 pg/ml
Sensitivity < 2 pg/ml
Storage and Expiration: Store at 2-8℃ for 4 months, or at -20℃ for 8 months.
Application: For quantitative detection of BMP-2 in serum, plasma, body fluids, tissue lysates or cell culture supernates.
1. One 96-well plate pre-coated with anti-Mouse BMP-2 antibody
2. Lyophilized BMP-2 standards: 2 tubes (10 ng / tube)
3. Sample / Standard diluent buffer: 30 ml
4. Biotin conjugated anti-Mouse BMP-2 antibody (Concentrated): 130 μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130 μl. Dilution: 1:100
7. ABC diluent buffer: 12 ml
8. TMB substrate: 10 ml
9. Stop solution: 10 ml
10. Wash buffer (25X): 30 ml
Note: Reconstitute standards and test samples with Kit Component 3.
Swiss Port: P21274
Gene ID: 12156