Overview

Introduction
Bone morphogenetic protein 2 (BMP-2) belongs to the TGF-β superfamily of proteins. Like other family members, it plays an important role in the development of bone and cartilage. It has been demonstrated to potently induce osteoblast differentiation in a variety of cell types.BMP-2 has an ability to induce the formation of ectopic cartilage when implanted subcutaneously.

Principle of the Assay  
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BMP-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BMP-2 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BMP-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BMP-2 can be calculated.

Range: 62.5 pg/ml-4000 pg/ml

Sensitivity < 2 pg/ml

Storage and Expiration: Store at 2-8℃ for 4 months, or at -20℃ for 8 months.

Application: For quantitative detection of BMP-2 in serum, plasma, body fluids, tissue lysates or cell culture supernates.

Kit components
1.     One 96-well plate pre-coated with anti-Rat BMP-2 antibody
2.     Lyophilized BMP-2 standards: 2 tubes (10 ng / tube)                    
3.     Sample / Standard diluent buffer: 30 ml
4.     Biotin conjugated anti-Rat BMP-2 antibody (Concentrated): 130 μl. Dilution: 1:100
5.     Antibody diluent buffer: 12 ml
6.     Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130 μl. Dilution: 1:100
7.     ABC diluent buffer: 12 ml
8.     TMB substrate: 10 ml
9.     Stop solution: 10 ml
10.   Wash buffer (25X): 30 ml
Note: Reconstitute standards and test samples with Kit Component 3.