The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. EGFR and its ligands function in diverse cellular functions including cell proliferation, differentiation, motility, and survival. EGFR signaling is important for the development of many tissues, including skin, lungs, intestines, and the craniofacial skeleton.
Principle of the Assay
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- EGFR polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- EGFR polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the EGFR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of EGFR can be calculated.
Range: 156 pg/ml-10000 pg/ml
Sensitivity < 1 pg/ml
Storage and Expiration: Store at 2-8℃ for 4 months, or at -20℃ for 8 months.
Application: For quantitative detection of EGFR in human serum, plasma, body fluids, tissue lysates or cell culture supernates
1. One 96-well plate pre-coated with anti-human EGFR antibody
2. Lyophilized EGFR standards: 2 tubes (10ng / tube)
3. Sample / Standard diluent buffer: 30ml
4. Biotin conjugated anti-Human EGFR antibody (Concentrated): 130μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
7. ABC diluent buffer: 12ml
8. TMB substrate: 10ml
9. Stop solution: 10ml
10. Wash buffer (25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
Swiss Port: P00533
Gene ID: 1956
Please check datasheet for protocol.