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beta HCG Mouse ELISA Kit
Overview
Introduction
Human chorionic gonadotropin (CG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. It is a glycoprotein composed of 237 amino acids with a molecular mass of 25.7 kDa. It is heterodimeric, with an α (alpha) subunit identical to that of luteinizing hormone (LH), follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and β (beta) subunit that is unique to hCG. β subunit is secreted by some cancers including seminoma, choriocarcinoma, germ cell tumors, hydatidiform mole formation, teratoma with elements of choriocarcinoma, and islet cell tumor. Combined with alpha-fetoprotein, β-HCG is an excellent tumor marker for the monitoring of germ cell tumors.
Principle of the Assay
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-beta HCG monoclonal antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-beta HCG polyclonal antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the beta HCG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of beta HCG can be calculated.
Principle of the Assay
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-beta HCG monoclonal antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-beta HCG polyclonal antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the beta HCG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of beta HCG can be calculated.
Range: 0 mIU/ml - 240 mIU/ml
Sensitivity < 2 mIU/ml
Specificity No cross react with other cytokines
Storage and Expiration: Store at 4℃ for 8 months.
Application: For quantitative detection of beta HCG in mouse serum or urine.
Kit components
1. One 96-well plate pre-coated with anti-mouse beta HCG antibody
2. Liquid beta HCG standards: 6 tubes (0 mIU/ml, 8 mIU/ml, 16 mIU/ml, 40 mIU/ml, 100 mIU/ml, 240 mIU/ml)
3. HRP conjugated anti-mouse beta HCG antibody (RTU): 6 ml
4. TMB substrate A: 7 ml
5. TMB substrate B: 7 ml
6. Stop solution: 7 ml
7. Sample diluent buffer: 30 ml
8. Wash buffer (25X): 30 ml
Gene Information
OMIM: 118860
Other Information
Please check datasheet for protocol.
