beta HCG Human ELISA Kit
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Principle of the Assay
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-beta HCG monoclonal antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-beta HCG polyclonal antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the beta HCG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of beta HCG can be calculated.
Range: 0 mIU/ml - 240 mIU/ml
Sensitivity < 2 mIU/ml
Specificity No cross react with other cytokines
Storage and Expiration: Store at 4℃ for 8 months.
Application: For quantitative detection of beta HCG in Human serum or urine.
1. One 96-well plate pre-coated with anti-Human beta HCG antibody
2. Liquid beta HCG standards: 6 tubes (0 mIU/ml, 8 mIU/ml, 16 mIU/ml, 40 mIU/ml, 100 mIU/ml, 240 mIU/ml)
3. HRP conjugated anti-Human beta HCG antibody (RTU): 6 ml
4. TMB substrate A: 7 ml
5. TMB substrate B: 7 ml
6. Stop solution: 7 ml
7. Urine diluent buffer (10 X): 15 ml
8. Wash buffer (25X): 30 ml
Swiss Port: P01233
Please check datasheet for protocol.