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C-Reactive Protein, CRP Human ELISA Kit
Overview
Introduction
C-reactive protein (CRP) is a protein found in the blood, the levels of which rise in response to inflammation. The high-sensitivity C-reactive protein (hs-CRP) is a marker of inflammation, and in the detection of patients at increased risk for cardiovascular disease. hs-CRP is an independent predictor of future risk for cardiovascular events among healthy individuals, as well as among patients with acute coronary syndromes. In addition, because half of all cardiovascular events occur in persons with low to average levels of low-density lipoprotein cholesterol, hs-CRP may aid in identifying patients at high risk for a first cardiovascular event who might otherwise be missed by lipid screening alone. Thus, hs-CRP is a potential adjunct for global risk assessment in the primary prevention of cardiovascular disease.
Principle of the Assay
This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti- CRP antibody was pre-coated onto 96-well plates. And the HRP conjugated anti- CRP antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CRP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CRP can be calculated.
Range: 150 μg/L - 1800μg/L
Sensitivity：15μg/L
Storage and Expiration: Store at 2-8℃ for 6 months.
Application: For quantitative detection of CRP in human serum, plasma, cell culture supernatant, saliva, urine or tissue samples.
Kit components
1. One 96-well plate pre-coated with anti-human CRP antibody
2. Standard: 0.5ml (2700 μg/L)
3. Standard diluent buffer: 1.5 ml
4. Wash buffer (30×): 20 ml. Dilution: 1:30
5. Sample diluent buffer: 6 ml
6. HRP conjugated anti-human CRP antibody (RTU): 6ml
7. Stop solution: 6 ml
8. TMB substrate A: 6ml
9. TMB substrate B: 6ml
10. Plate sealer: 2
11. Hermetic bag: 1
C-reactive protein (CRP) is a protein found in the blood, the levels of which rise in response to inflammation. The high-sensitivity C-reactive protein (hs-CRP) is a marker of inflammation, and in the detection of patients at increased risk for cardiovascular disease. hs-CRP is an independent predictor of future risk for cardiovascular events among healthy individuals, as well as among patients with acute coronary syndromes. In addition, because half of all cardiovascular events occur in persons with low to average levels of low-density lipoprotein cholesterol, hs-CRP may aid in identifying patients at high risk for a first cardiovascular event who might otherwise be missed by lipid screening alone. Thus, hs-CRP is a potential adjunct for global risk assessment in the primary prevention of cardiovascular disease.
Principle of the Assay
This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti- CRP antibody was pre-coated onto 96-well plates. And the HRP conjugated anti- CRP antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CRP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CRP can be calculated.
Range: 150 μg/L - 1800μg/L
Sensitivity：15μg/L
Storage and Expiration: Store at 2-8℃ for 6 months.
Application: For quantitative detection of CRP in human serum, plasma, cell culture supernatant, saliva, urine or tissue samples.
Kit components
1. One 96-well plate pre-coated with anti-human CRP antibody
2. Standard: 0.5ml (2700 μg/L)
3. Standard diluent buffer: 1.5 ml
4. Wash buffer (30×): 20 ml. Dilution: 1:30
5. Sample diluent buffer: 6 ml
6. HRP conjugated anti-human CRP antibody (RTU): 6ml
7. Stop solution: 6 ml
8. TMB substrate A: 6ml
9. TMB substrate B: 6ml
10. Plate sealer: 2
11. Hermetic bag: 1
Other Information
Please check datasheet for protocol.
