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Catechol-O-Methyl transferase, COMT Human ELISA Kit

Catalog #BYEK3236
In stockYes
Datasheet MSDS
Catechol-O-methyltransferase (COMT) is one of several enzymes that degrade catecholamines (such as dopamine, epinephrine, and norepinephrine), catecholestrogens, and various drugs and substances having a catechol structure.  In humans, catechol-O-methyltransferase protein is encoded by the COMT gene. COMT polymorphism, and by implication cortical dopamine degradation, influences the expression of a trans-diagnostic compulsivity phenotype, even accounting for possible confounding effects of impulsivity. The function of the catechol-O-methyl transferase (COMT) gene has been found to be associated with human fearful or anxious emotions, and the COMT:p.Val158Met polymorphism locus is significantly related to anxious behavior.

Principle of the Assay  

This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti- COMT antibody was pre-coated onto 96-well plates. And the HRP conjugated anti- COMT antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the COMT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of COMT can be calculated.

Range: 250 pg/ml- 3000 pg/ml

Sensitivity:10 pg/ml

Storage and Expiration: Store at 2-8℃ for 6 months. 

Application: For quantitative detection of COMT in human serum, plasma, tissue homogenate or cell culture supernatant.

Kit components
1. One 96-well plate pre-coated with anti- human COMT antibody
2. Standard: 0.5ml (4500 pg/ml)
3. Standard diluent buffer: 1.5 ml
4. Wash buffer (30×): 20 ml. Dilution: 1:30
5. Sample diluent buffer: 6 ml
6. HRP conjugated anti- human COMT antibody (RTU): 6ml
7. Stop solution: 6 ml
8. TMB substrate A:  6ml
9. TMB substrate B:  6ml
10. Plate sealer:   2
11. Hermetic bag:  1

Other Information

Please check datasheet for protocol.