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Horse Interleukin 10, IL-10 ELISA Kit

Catalog #BYEK3448
In stockyes
Datasheet MSDS

Catalog No.: BYEK3448

Size: 96T

Range: 12.5 pg/ml- 200 pg/ml 

Sensitivity: 1.25 pg/ml

Storage and Expiration: Store at 2-8℃ for 6 months. 

Application: For quantitative detection of IL-10 in horse serum, plasma, tissue homogenate or cell culture supernatant.

Interleukin-10 (IL-10 or IL10) also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, IL-10 is encoded by the IL10 gene, which is located on chromosome 1 and comprises 5 exons. The IL-10 protein is a homodimer, each of its subunits is 178-amino-acid long. It is primarily produced by monocytes. IL-10 is a cytokine with pleiotropic effects in immunoregulation and inflammation. It could downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production.

Principle of the Assay  
This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti- IL-10 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti- IL-10 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-10 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-10 can be calculated.

Kit components
1. One 96-well plate pre-coated with anti- horse IL-10 antibody
2. Standard: 0.3 ml × 6 tubes (0 pg/ml, 12.5 pg/ml, 25 pg/ml, 50 pg/ml, 100 pg/ml, 200 pg/ml)
3. Wash buffer (20×): 25 ml. Dilution: 1:20
4. Sample diluent buffer: 6 ml
5. HRP conjugated anti- horse IL-10 antibody (RTU): 10 ml
6. Stop solution: 6 ml
7. TMB substrate A:  6ml
8. TMB substrate B:  6ml
9. Plate sealer:   2
10. Hermetic bag:  1

Material Required But Not Provided
1. 37℃ incubator
2. Microplate reader (wavelength: 450nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5ml of Eppendorf tubes
7. Absorbent filter papers
8. Plastic or glass container with volume of above 1L

Other Information

Please check datasheet for protocol.