Polyclonal Anti- Autoimmune regulator, Aire Antibody

Catalog#  BPA1005

Lot # Check on the product label

Size 100 μg

Isotype IgG

Host Rabbit

Reactivity Mouse

Product Form Liquid

Purification Immunogen affinity purified

Immunogen 

A synthetic peptide (conjugated with KLH) corresponding to the region of mouse Aire.

Recommend Application

Western Blot, WB (1:1000)

Immunohistochemistry, IHC-P (1:50)

Immunocytochemistry, ICC (1:200)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

The autoimmune regulator (AIRE) is a protein with a calculated molecular mass of 57,723 DA.  AIRE is a transcription factor expressed in the medulla of the thymus. The autoimmune regulator is mutated in the rare autoimmune syndrome Autoimmune Polyendocrinopathy Syndrome type 1 (APS-1), also known as Autoimmune Polyendocrinopathy - Candidiasis - Ectodermal Dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis.

Reference

1. Nagamine, K., Peterson, P., Scott, H. S., Kudoh, J., Minoshima, S., Heino, M., Krohn, K. J. E., Lalioti, M. D., Mullis, P. E., Antonarakis, S. E., Kawasaki, K., Asakawa, S., Ito, F., Shimizu, N. Positional cloning of the APECED gene. Nature Genet. 17: 393-398, 1997.

2. Faiyaz-Ul-Haque, M., Bin-Abbas, B., Al-Abdullatif, A., Abdullah Abalkhail, H., Toulimat, M., Al-Gazlan, S., Almutawa, A. M., Al-Sagheir, A., Peltekova, I., Al-Dayel, F., Zaidi, S. H. E. Novel and recurrent mutations in the AIRE gene of autoimmune polyendocrinopathy syndrome type 1 (APS1) patients. Clin. Genet. 76: 431-440, 2009.