Human IL-18 ELISA Kit
Size: 96T
Range: 62.5 pg/ml - 4000 pg/ml
Sensitivity < 8 pg/ml
Application: For quantitative detection of IL-18 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
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Price: $460.00
Catalog No.: BEK1125
Size: 96T
Range: 62.5 pg/ml - 4000 pg/ml
Sensitivity < 8 pg/ml
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Application: For quantitative detection of IL-18 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction
Interleukin-18 (IL18), also known as interferon-gamma inducing factor, in humans is encoded by the IL18 gene, this gene contains 6 exons, and maps to 11q22.2-q22.3. It is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. It works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). IL-18 has been implicated as an inflammatory mediator of Hashimoto's thyroiditis, the most common cause of autoimmune hypothyroidism. IL-18 is also able to induce severe inflammatory reactions, which suggests its role in certain inflammatory disorders.
Principle of the Assay
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-18 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-18 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-18 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-18 can be calculated.
Kit components
- One 96-well plate pre-coated with anti-Human IL-18 antibody
- Lyophilized Human IL-18 standards: 2 tubes (10 ng / tube)
- Sample / Standard diluent buffer: 30ml
- Biotin conjugated anti-Human IL-18 antibody (Concentrated): 130μl. Dilution: 1:100
- Antibody diluent buffer: 12ml
- Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
- ABC diluent buffer: 12ml
- TMB substrate: 10ml
- Stop solution: 10ml
- Wash buffer (25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
Material Required But Not Provided
- 37℃ incubator
- Microplate reader (wavelength: 450nm)
- Precise pipette and disposable pipette tips
- Automated plate washer
- ELISA shaker
- 1.5ml of Eppendorf tubes
- Plate cover
- Absorbent filter papers
- Plastic or glass container with volume of above 1L