Mouse LOX1 ELISA Kit

Catalog No.: BEK1139

Size: 96T

Range: 31.2 pg/ml-2000 pg/ml

Sensitivity < 1 pg/ml

Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months. 

Application: For quantitative detection of LOX1 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Introduction

Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) also known as lectin-type oxidized LDL receptor 1 (LOX-1) is a 270-amino acid protein that in humans is encoded by the OLR1 gene, which spans approximately 15 kb and contains 6 exons. LOX1 is expressed in vascular-rich organs but not in lymphocytes, and it belongs to the C-type lectin superfamily. This protein binds, internalizes and degrades oxidized low-density lipoprotein. It may be involved in the regulation of Fas-induced apoptosis, and it may also play a role as a scavenger receptor. Mutations of the OLR1 gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease.

Principle of the Assay  

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-LOX1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-LOX1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LOX1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LOX1 can be calculated.

Kit components

1. One 96-well plate pre-coated with anti-Mouse LOX1 antibody
2. Lyophilized Mouse LOX1 standards: 2 tubes (10 ng / tube)                     
3. Sample / Standard diluent buffer: 30ml
4. Biotin conjugated anti-Mouse LOX1 antibody (Concentrated): 130μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
7. ABC diluent buffer: 12ml
8. TMB substrate: 10ml
9. Stop solution: 10ml
10. Wash buffer (25X): 30ml

Note: Reconstitute standards and test samples with Kit Component 3.

Material Required But Not Provided

1. 37℃ incubator
2. Microplate reader (wavelength: 450nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5ml of Eppendorf tubes
7. Plate cover
8. Absorbent filter papers
9. Plastic or glass container with volume of above 1L