Mouse L-Selectin ELISA Kit
Size: 96T
Range: 62.5 pg/ml-4000 pg/ml
Sensitivity < 5 pg/ml
Application: For quantitative detection of L-Selectin in serum, plasma, body fluids, tissue lysates or cell culture supernates.
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Price: $350.00
Catalog No.: BEK1141
Size: 96T
Range: 62.5 pg/ml-4000 pg/ml
Sensitivity < 5 pg/ml
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Application: For quantitative detection of L-Selectin in serum, plasma, body fluids, tissue lysates or cell culture supernates.
Introduction
L-selectin, also known as CD62L, is a cell adhesion molecule found on lymphocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4-binding proteins. L-selectin acts as a "homing receptor" for lymphocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to lymphocytes expressing L-selectin, slowing lymphocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. It may also play a role in neutrophil adhesion to endothelium at sites of inflammation.
Principle of the Assay
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- L-Selectin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- L-Selectin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the L-Selectin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of L-Selectin can be calculated.
Kit components
- One 96-well plate pre-coated with anti-Mouse L-Selectin antibody
- Lyophilized L-Selectin standards: 2 tubes (10 ng / tube)
- Sample / Standard diluent buffer: 30ml
- Biotin conjugated anti-Mouse L-Selectin antibody (Concentrated): 130μl. Dilution: 1:100
- Antibody diluent buffer: 12ml
- Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
- ABC diluent buffer: 12ml
- TMB substrate: 10ml
- Stop solution: 10ml
- Wash buffer (25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
Material Required But Not Provided
- 37℃ incubator
- Microplate reader (wavelength: 450nm)
- Precise pipette and disposable pipette tips
- Automated plate washer
- ELISA shaker
- 1.5ml of Eppendorf tubes
- Plate cover
- Absorbent filter papers
- Plastic or glass container with volume of above 1L