Human MCP-2 ELISA Kit

Catalog No.: BEK1144

Size: 96T

Range: 30 pg/ml-1000 pg/ml

Storage and Expiration: Store at 2-8℃ for 6 months. 

Application: For quantitative detection of MCP-2 in human serum, plasma, urine, cell culture supernatant or tissue samples.

Introduction

Monocyte chemotactic protein 2 (MCP-2), also known as Chemokine (C-C motif) ligand 8 (CCL8), is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes. CCL8 is a small cytokine belonging to the CC chemokine family. It is a potent inhibitor of HIV-1 by virtue of its high-affinity binding to the receptor CCR5, one of the major co-receptors for HIV-1. It elicits its effects by binding to several different cell surface receptors called chemokine receptors. These receptors include CCR1, CCR2B and CCR5.

Principle of the Assay  

This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-MCP-2 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-MCP-2 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MCP-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MCP-2 can be calculated.

Kit components

1. One 96-well plate pre-coated with anti-human MCP-2 antibody

2. Standard: 0.5ml (1350pg /mL)

3. Standard diluent buffer: 1.5 ml

4. Wash buffer (30×): 20 ml. Dilution: 1:30

5. Sample diluent buffer: 6 ml

6. HRP conjugated anti-human MCP-2 antibody (RTU): 6ml

7. Stop solution: 6 ml

8. TMB substrate A:  6ml

9. TMB substrate B:  6ml

10. Plate sealer:   2

11. Hermetic bag:  1

Material Required But Not Provided

1. 37℃ incubator
2. Microplate reader (wavelength: 450nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5ml of Eppendorf tubes
7. Absorbent filter papers
8. Plastic or glass container with volume of above 1L