Human MMP-1 ELISA Kit
Size: 96T
Range: 156 pg/ml-10,000 pg/ml
Sensitivity < 10 pg/ml
Application: For quantitative detection of MMP-1 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
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Price: $350.00
Catalog No.: BEK1155
Size: 96T
Range: 156 pg/ml-10,000 pg/ml
Sensitivity < 10 pg/ml
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Application: For quantitative detection of MMP-1 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Introduction
Matrix metalloproteinase-1 (MMP-1), also known as interstitial collagenase and fibroblast collagenase, is a member of the matrix metalloproteinases family. Matrix metalloproteinases are zinc-dependent proteases that degrade extracellular matrix proteins. MMP-1 is structurally formed by different proteic building blocks. The Catalytic Domain of MMP-1 is composed of five highly twisted β-strands (sI-sV), three α-helix (hA-hC) and a total of eight loops, enclosing a total of five metal ions, three Ca2+ and two Zn2+, one of which with catalytic role. MMP1 in the stromal-tumor microenvironment can alter the behavior of cancer cells through PAR1 to promote cell migration and invasion. The biologic implications of MMP1 acting as a molecular ratchet tethered to the cell surface suggested novel mechanisms for its role in tissue remodeling and cell-matrix interaction.
Principle of the Assay
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MMP-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MMP-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MMP-1 can be calculated.
Kit components
- One 96-well plate pre-coated with anti-Human MMP-1 antibody
- Lyophilized Human MMP-1 standards: 2 tubes (10 ng / tube)
- Sample / Standard diluent buffer: 30ml
- Biotin conjugated anti-human MMP-1 antibody (Concentrated): 130μl. Dilution: 1:100
- Antibody diluent buffer: 12ml
- Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
- ABC diluent buffer: 12ml
- TMB substrate: 10ml
- Stop solution: 10ml
- Wash buffer(25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
Material Required But Not Provided
- 37℃ incubator
- Microplate reader (wavelength: 450nm)
- Precise pipette and disposable pipette tips
- Automated plate washer
- ELISA shaker
- 1.5ml of Eppendorf tubes
- Plate cover
- Absorbent filter papers
- Plastic or glass container with volume of above 1L