Catalog No.: BEK1187
Size: 96T
Range: 31.2 pg/ml-2000 pg/ml
Sensitivity < 3 pg/ml
Application: For quantitative detection of PDGF-AB in rat serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Price: $300.00


Catalog No.: BEK1187

Size: 96T

Range: 31.2 pg/ml-2000 pg/ml

Sensitivity < 3 pg/ml

Storage and Expiration: Store at 2-8℃ for 6 months, or at -20 for 12 months. 

Application: For quantitative detection of PDGF-AB in rat serum, plasma, body fluids, tissue lysates or cell culture supernatants.


Platelet-derived growth factor (PDGF) was one of the first growth factors characterized. and also a potent mitogen for cells of mesenchymal origin, including smooth muscle cells and glial cells. It regulates cell growth and division, In particular, it plays a significant role in blood vessel formation (angiogenesis). It is produced by a plethora of cells, and synthesized, stored, released by platelets upon activation. In chemical terms, PSGF is dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB). It is found that initial PDGF-AB interaction with the alpha PDGFR induces conformational changes in the ligand or receptor that facilitates efficient recruitment of beta PDGFR by this PDGF isoform.

Principle of the Assay  

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PDGF-AB polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PDGF-AB polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PDGF-AB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PDGF-AB can be calculated.

Kit components

  1. One 96-well plate pre-coated with anti-Rat PDGF-AB antibody
  2. Lyophilized Rat PDGF-AB standards: 2 tubes (10  ng / tube)                     
  3. Sample / Standard diluent buffer: 30ml
  4. Biotin conjugated anti-Rat PDGF-AB antibody (Concentrated): 130μl. Dilution: 1:100
  5. Antibody diluent buffer: 12ml
  6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
  7. ABC diluent buffer: 12ml
  8. TMB substrate: 10ml
  9. Stop solution: 10ml
  10. Wash buffer(25X): 30ml

Note: Reconstitute standards and test samples with Kit Component 3.

Material Required But Not Provided

  1. 37℃ incubator
  2. Microplate reader (wavelength: 450nm)
  3. Precise pipette and disposable pipette tips
  4. Automated plate washer
  5. ELISA shaker
  6. 1.5ml of Eppendorf tubes
  7. Plate cover
  8. Absorbent filter papers
  9. Plastic or glass container with volume of above 1L