Polyclonal Anti- Sex determining region Y-box 2, SOX2

Catalog#  BPA1112

Lot # Check on the product label

Size 100 μg

Isotype IgG

Host Rabbit

Reactivity Human, mouse

Product Form Liquid

Purification Immunogen affinity purified

Immunogen A synthetic peptide (conjugated with KLH) corresponding to the region of SOX2.

Recommend Application

Western Blot, WB (1:200-1:500)

Immunocytochemistry, ICC (1:10-1:20)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

SRY (sex determining region Y)-box 2, also known as SOX2, is a transcription factor that is essential to maintain self-renewal of undifferentiated embryonic stem cells. SOX2 gene encodes a 317-amino acid protein, and lies in an intron of the SOX2OT (SOX2 overlapping transcript) gene with single-exon. SOX2 is expressed early in the embryonic stem cells of the blastocyst and later in the neural stem cells. It is a member of the SOX family of proteins that carry a DNA-binding high-mobility group (HMG) domain and additional domains that regulate embryonic development and cell fate determinations. Mutations in SOX2 can cause not only anophthalmia, but also aplasia of the optic nerve, chiasm and optic tract, as well as modest bilateral sensorineural hearing loss, and global developmental delay, underscoring the importance of SOX2 in early human eye and brain development. And the ectopic expression of SOX2 may be related to abnormal differentiation of colorectal cancer cells.

Reference

1. Stevanovic, M., Zuffardi, O., Collignon, J., Lovell-Badge, R., Goodfellow, P. The cDNA sequence and chromosomal location of the human SOX2 gene. Mammalian Genome 5: 640-642, 1994.

2. Fantes, J., Ragge, N. K., Lynch, S.-A., McGill, N. I., Collin, J. R. O., Howard-Peebles, P. N., Hayward, C., Vivian, A. J., Williamson, K., van Heyningen, V., FitzPatrick, D. R. Mutations in SOX2 cause anophthalmia. Nature Genet. 33: 461-462, 2003.

3. Hagstrom, S. A., Pauer, G. J. T., Reid, J., Simpson, E., Crowe, S., Maumenee, I. H., Traboulsi, E. I. SOX2 mutation causes anophthalmia, hearing loss, and brain anomalies. Am. J. Med. Genet. 138A: 95-98, 2005.

4. Tani, Yasuyo; Akiyama, Y; Fukamachi, H; Yanagihara, K; Yuasa, Y (2007). "Transcription factor SOX2 up-regulates stomach-specific pepsinogen A gene expression". J Cancer Res Clin Oncol 133 (4): 263–9.