Monoclonal Anti- 14-3-3 beta / alpha Antibody

Catalog# BMA1001

Lot # Check on the product label

Size 100 μg

Isotype IgG2a

Clone # N14

Host Mouse

Reactivity Human, mouse, rat

Product Form Liquid

Purification Protein A purified

Immunogen 

A synthetic peptide (conjugated with KLH) corresponding to the C-terminal of mouse 14-3-3 beta / alpha.

Recommend Application

Western Blot, WB (1:500-1000)

Immunohistochemistry, IHC-P (1:100)

Immunocytochemistry, ICC (1:50)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

14-3-3 proteins are a family of conserved regulatory molecules expressed in all eukaryotic cells. They have the ability to bind a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors. More than 100 signaling proteins have been reported as 14-3-3 ligands, 14-3-3 b/a is one of them. They play important roles in a wide range of vital regulatory processes, such as mitogenic signal transduction, apoptotic cell death, and cell cycle control. Elevated amounts of 14-3-3 proteins are found in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease.

Reference

1. Takahashi H, Iwata T, Kitagawa Y et al. (November 1999). "Increased levels of epsilon and gamma isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease". Clin. Diagn. Lab. Immunol. 6 (6): 983–5.