Monoclonal Anti- Albumin Bovine, BSA Antibody

Catalog# BMA1003

Lot # Check on the product label

Size 100 μg

Isotype IgG1

Clone # D8-2

Host Mouse

Reactivity Bovine

Product Form Liquid

Purification Protein A purified

Immunogen Protein

Recommend Application

Western Blot, WB (1:200-500)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Serum albumin (also known as BSA or "Fraction V") is a serum albumin protein derived from cows. It is composed of 585 amino acid residues, and its molecular weight is about 66.5KDa. The BSA is the main protein of plasma, and it has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. It is often used as a protein concentration standard, and it has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry.

Reference

1. Peters T (1975). Putman FW. ed. The Plasma Proteins. Academic Press. pp. 133–181.

2. Brown J.R.; "Structure of serum albumin: disulfide bridges."; Fed. Proc. 33:1389-1389(1974).

3. Carraway R.E., Mitra S.P., Cochrane D.E.; "Structure of a biologically active neurotensin-related peptide obtained from pepsin-treated albumin(s)."; J. Biol. Chem. 262:5968-5973(1987).