Monoclonal Anti- B-cell lymphoma 2, Bcl-2 Antibody

Catalog# BMA1007

Lot # Check on the product label

Size 100 μg

Isotype IgG2b

Clone # D24-5

Host Mouse

Reactivity Human, mouse

Product Form Liquid

Purification Protein A purified

Immunogen 

Recombinant protein within human BCL-2.

Recommend Application

Western Blot,WB (1:50)

Immunohistochemistry, IHC-P (1:100)

Immunocytochemistry, ICC (1:100-200)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Bcl-2 (B-cell lymphoma 2) is the founding member of the Bcl-2 family of apoptosis regulator proteins encoded by the BCL2 gene. Bcl-2 is an important regulator of photoreceptor cell death in retinal degenerations. The bcl-2 protooncogene, which protects various cell types from apoptotic cell death, is expressed in the developing and adult nervous system. Bcl-2 may play an important role in survival of neurons both during development and throughout adult life.

Reference

1. Tsujimoto Y, Finger LR, Yunis J, Nowell PC, Croce CM (November 1984). "Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation". Science 226 (4678): 1097–99.

2. Cleary ML, Smith SD, Sklar J (October 1986). "Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation". Cell 47 (1): 19–28.

3. Chen, J., Flannery, J. G., LaVail, M. M., Steinberg, R. H., Xu, J., Simon, M. I. Bcl-2 overexpression reduces apoptotic photoreceptor cell death in three different retinal degenerations. Proc. Nat. Acad. Sci. 93: 7042-7047, 1996.

4. Farlie, P. G., Dringen, R., Rees, S. M., Kannourakis, G., Bernard, O. bcl-2 transgene expression can protect neurons against developmental and induced cell death. Proc. Nat. Acad. Sci. 92: 4397-4401, 1995.