Human TGF-β2 ELISA Kit

Catalog No.: BEK1204

Size: 96T

Range: 10 pg/ml-800 pg/ml

Storage and Expiration: Store at 2-8℃ for 6 months. 

Application: For quantitative detection of TGF-β2 in human serum, plasma, urine, cell culture supernatant or tissue samples.

Introduction

Transforming growth factor-beta 2 (TGF-β2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. It is known to suppress the effects of interleukin dependent T-cell tumors. It is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma. In 2004, Gottanka found that TGF-β2 reduced outflow facility when perfused into cultured human anterior segments. Furthermore, TGF-β2 affected the extracellular matrix of the trabecular meshwork in a manner that was consistent with the observed reduction in outflow facility.

Principle of the Assay  

This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-TGF-β2 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-TGF-β2 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TGF-β2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TGF-β2 can be calculated.

Kit components

1. One 96-well plate pre-coated with anti-human TGF-β2 antibody

2. Standard:  0.5ml (900 pg /mL)

3. Standard diluent buffer:  1.5 ml

4. Wash buffer (30×):  20 ml. Dilution: 1:30

5. Sample diluent buffer: 6 ml

6. HRP conjugated anti-human TGF-β2 antibody (RTU): 6ml

7. Stop solution: 6 ml

8. TMB substrate A:  6ml

9. TMB substrate B:  6ml

10. Plate sealer:   2

11. Hermetic bag:  1

Material Required But Not Provided

1. 37℃ incubator
2. Microplate reader (wavelength: 450nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5ml of Eppendorf tubes
7. Absorbent filter papers
8. Plastic or glass container with volume of above 1L