Monoclonal Anti- Cyclin E2 Antibody

Catalog# BMA1014

Lot # Check on the product label

Size 100 μg

Isotype IgG1

Clone # N24-5

Host Mouse

Reactivity

Human, mouse

Product Form Liquid

Purification Protein A purified

Immunogen 

A synthetic peptide (conjugated with KLH) corresponding to the C-terminal of Cyclin E2.

Recommend Application

Western Blot, WB (1:500-2000)

Immunohistochemistry, IHC-P (1:100)

Immunocytochemistry, ICC (1:50)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Cyclin E2 is a member of the cyclin family, It is a predicted 404-amino acid protein which contains a cyclin box motif and it is 47% identical to cyclin E1. It is expressed in several normal human tissues, with the highest levels in testis, thymus, and brain. Gudas et al. (1999) found that, like cyclin E1, the human cyclin E2 gene complemented a G1 cyclin defect in S.  cerevisiae. Cyclin E2 expressed in mammalian cells associated with CDK2 in a functional kinase complex that was inhibited by both KIP1 and CIP1. 

Reference

1. Gudas, J. M., Payton, M., Thukral, S., Chen, E., Bass, M., Robinson, M. O., Coats, S. Cyclin E2, a novel G1 cyclin that binds Cdk2 and is aberrantly expressed in human cancers. Molec. Cell. Biol. 19: 612-622, 1999.