Monoclonal Anti- DNA-dependent protein kinase, catalytic subunit, DNA-PKcs / PRKDC Antibody

Catalog# BMA1017  

Lot # Check on the product label

Size 100 μg

Isotype IgG2a

Clone # N9

Host Mouse

Reactivity Human, mouse

Product Form Liquid

Purification Protein A purified

Immunogen 

Recombinant DNA-PKcs protein.

Recommend Application

Western Blot, WB (1:500)

Immunohistochemistry, IHC-P (1:100)

Immunocytochemistry, ICC (1:50)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

DNA-dependent protein kinase, catalytic subunit, also known as DNA-PKcs, is an enzyme that belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Anderson and Lees-Miller (1992) noted that DNA-PK had been shown in vitro to phosphorylate several transcription factors, suggesting that it functions in cell homeostasis by modulating transcription. It acts as a molecular sensor for DNA damage, and it may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage.

Reference

1. Anderson, C. W., Lees-Miller, S. P. The nuclear serine/threonine protein kinase DNA-PK. Crit. Rev. Eukaryot. Gene Expr. 2: 283-314, 1992.

2. Yavuzer U., Smith G.C.M., Bliss T., Werner D., Jackson S.P. "DNA end-independent activation of DNA-PK mediated via association with the DNA-binding protein C1D." Genes Dev. 12:2188-2199(1998)

3. Soubeyrand S., Pope L., Pakuts B., Hache R.J. "Threonines 2638/2647 in DNA-PK are essential for cellular resistance to ionizing radiation." Cancer Res. 63:1198-1201(2003)