Monoclonal Anti- Glutathione S-transferase, GST Antibody

Catalog# BMA1022

Lot # Check on the product label

Size 100 μg

Isotype IgG1

Clone # F8

Host Mouse

Reactivity All species

Product Form Liquid

Purification Protein A purified

Immunogen 

Recombinant GST protein expressed by pGEX.

Recommend Application

Western Blot, WB (1:1000-2000)

ELISA (1:5,000-1:10,000)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Glutathione S-transferases are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. Genetic polymorphisms in glutathione S-transferase and its altered expression and activity are associated with oxidative DNA damage and also damage of the kidney. Subsequently the individual's risk of cancer susceptibility increases.

Reference

Khan MA, Tania M, Zhang D, Chen H (2010). "Antioxidant enzymes and cancer". Chin J Cancer Res 22 (2): 87–92.