Monoclonal Anti- Kinase D-interacting substrate 220, Kidins 220 Antibody

Catalog# BMA1027

Lot # Check on the product label

Size 100 μg

Isotype IgG1

Clone # K23

Host Mouse

Reactivity Human ,mouse

Product Form Liquid

Purification Protein A purified

Immunogen 

Recombinant Kidins 220 protein.

Recommend Application

Western Blot,WB (1:500-1:5,000)

Immunohistochemistry, IHC-P (1:20)

Immunocytochemistry, ICC (1:50)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Protein kinase D (PKD) is a serine/threonine kinase regulated by diacylglycerol signaling pathways with unique domain composition and enzymatic properties, still awaiting identification of its specific substrate(s). Kinase D-interacting substrate 220(Kidins220) is an enzyme that in humans is encoded by the KIDINS220 gene. It is an integral membrane protein selectively expressed in brain and neuroendocrine cells, where it concentrates at the tip of neurites. and it contains 11 ankyrin repeats and four transmembrane domains within the N-terminal region.

Reference

1. Iglesias T, Cabrera-Poch N, Mitchell MP, Naven TJ, Rozengurt E, Schiavo G (Jan 2001). "Identification and cloning of Kidins220, a novel neuronal substrate of protein kinase D". J Biol Chem 275 (51): 40048–56.