Monoclonal Anti- Lactate dehydrogenase A, LDH-A Antibody

Catalog# BMA1029

Lot # Check on the product label

Size 100 μg

Isotype IgG1

Clone # L46-2

Host Mouse

Reactivity

Human, mouse

Product Form Liquid

Purification Protein A purified

Immunogen 

A synthetic peptide (conjugated with KLH) corresponding to the N-terminal of human LDH-A.

Recommend Application

Western Blot,WB (1:100-200)

Immunohistochemistry, IHC-P (1:20)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Lactate dehydrogenase A, also known as LDHA, is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. It is encoded by the LDHA gene which contains 7 exons and spans about 12 kb. It catalyzes the conversion of L-lactate and NAD+ to pyruvate and NADH in the final step of anaerobic glycolysis. Mutations in LDHA have been linked to exertional myoglobinuria.

Reference

1. Chung, F.-Z., Tsujibo, H., Bhattacharyya, U., Sharief, F. S., Li, S. S.-L. Genomic organization of human lactate dehydrogenase-A gene. Biochem. J. 231: 537-541, 1985.

2. Tsujibo, H., Tiano, H. F., Li, S. S.-L. Nucleotide sequences of the cDNA and an intronless pseudogene for human lactate dehydrogenase-A isozyme. Europ. J. Biochem. 147: 9-15, 1985.