Monoclonal Anti- Neuropilin and tolloid like 1, NETO1 Antibody

Catalog# BMA1031

Lot # Check on the product label

Size 100 μg

Isotype IgM

Clone # N9-18

Host Mouse

Reactivity Human, mouse

Product Form Liquid

Purification Protein A purified

Immunogen 

Recombinant Neuropilin and tolloid like 1 protein.

Recommend Application

Western Blot,WB (1:500-5,000)

Immunohistochemistry, IHC-P (1:20)

Immunocytochemistry, ICC (1:50-200)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Neuropilin and tolloid-like protein 1 (NETO1) is encoded by NETO1 gene which contains 13 exons spanning more than 120 kb. It generates 3 isoforms. Isoform 1, produced by alternative usage of exon 5, encodes a 135-amino acid sequence with a classic signal peptide of 21 amino acids giving rise to a mature 114-amino acid protein with a calculated molecular mass of 15.4 kD. Isoforms 2 and 3, with start sites in exons 1a and 1b, respectively, encode distinct precursor proteins but result in a single mature protein of 511 identical amino acids with a calculated molecular mass of 57.8 kD. 

Reference

1. Stohr, H., Berger, C., Frohlich, S., Weber, B. H. F. A novel gene encoding a putative transmembrane protein with two extracellular CUB domains and a low-density lipoprotein class A module: isolation of alternatively spliced isoforms in retina and brain. Gene 286: 223-231, 2002.