Monoclonal Anti- P53 antibody

Catalog# BMA1033

Lot # Check on the product label

Size 100 μg

Isotype IgG2b

Clone # N33

Host Mouse

Reactivity Human

Product Form Liquid

Purification Protein A purified

Immunogen Recombinant human P53 protein.

Recommend Application

Western Blot,WB (1:500)

Immunohistochemistry, IHC-P (1:50-100)

Immunocytochemistry, ICC (1:50-100)

Flow Cytometry, Flow-Cyt (1:50-100)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide. 

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

P53 (also known as protein 53 or tumor protein 53), is a 393-amino acid protein, and its molecular weight is about 53KDa. It is crucial in multicellular organisms, where it regulates the cell cycle and, thus, functions as a tumor suppressor that is involved in preventing cancer. As such, P53 has been described as "the guardian of the genome" because of its role in conserving stability by preventing genome mutation. It is an important key element which controls cell cycle progression in response to cellular stress like DNA damage. Its ability to act as transcriptional activator or repressor links transcription and cell cycle control.

Reference

1. Vogelstein, B., Kinzler, K. W. X-rays strike p53 again. Nature 370: 174-175, 1994.

2. Read, A. P.; Strachan, T.. Human molecular genetics 2. New York: Wiley; 1999.

3. Schneider E., Montenarh M., Wagner P. "Regulation of CAK kinase activity by p53." Oncogene 17:2733-2741(1998)