Human Creatine Kinase MB, CK-MB ELISA Kit
Size: 96T
Range: 3.12 ng/ml-200 ng/ml
Sensitivity < 1 ng/ml
Application: For quantitative detection of CK-MB in human serum, plasma, tissue homogenates or cell culture supernatants.
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Price: $350.00
Catalog No.: BEK1248
Size: 96T
Range: 3.12 ng/ml-200 ng/ml
Sensitivity < 1 ng/ml
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Application: For quantitative detection of CK-MB in human serum, plasma, tissue homogenates or cell culture supernatants.
Introduction
Creatine kinase isoenzyme-MB (CK-MB) is a biomarker for detecting myocardial injury. It is a simple, widely available, and useful biomarker for predicting adverse in-hospital clinical outcomes in pulmonary embolism. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The CK-MB-to-total-CK ratio is an easily available indicator and could be clinically utilized as a primary screening tool for cancer. Higher ratio of CK-MB-to-total-CK was specifically associated with certain malignancies, like colorectal cancer, lung cancer and hepatocellular carcinoma, as well as some cancer-associated status factors such as advanced stage and liver metastasis.
Principle of the Assay
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CK-MB monoclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CK-MB monoclonal antibody was used as detection antibody. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CK-MB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CK-MB can be calculated.
Kit components
- One 96-well plate pre-coated with anti-human CK-MB antibody
- Lyophilized human CK-MB standards: 2 tubes (200 ng/tube)
- Sample / Standard diluent buffer: 30ml
- Biotin conjugated anti-human CK-MB antibody (Concentrated): 130μl. Dilution: 1:100
- Antibody diluent buffer: 12ml
- Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
- ABC diluent buffer: 12ml
- TMB substrate: 10ml
- Stop solution: 10ml
- Wash buffer(25X): 30ml
Note: Reconstitute standards and test samples with Kit Component 3.
Material Required But Not Provided
- 37℃ incubator
- Microplate reader (wavelength: 450nm)
- Precise pipette and disposable pipette tips
- Automated plate washer
- ELISA shaker
- 1.5ml of Eppendorf tubes
- Plate cover
- Absorbent filter papers
- Plastic or glass container with volume of above 1L