Ciliary Neurotrophic Factor (CNTF) Protein (Active)

Catalog# BRP1002

Lot # Check on the product label

Size 100 μg

Description

Recombinant full length human CNTF protein with molecular weight 22.8 kDa.

Synonyms HCNTF, CNTF, Ciliary Neurotrophic Factor

Source E. coli

Purity >95% by HPLC & SDS-PAGE

Formulation Lyophilized

Reconstitution

Reconstitute with sterile 18MΩ-cm H₂O.

Endotoxin level < 0.1 ng/μg (1 EU/μg) by LAL test

Dimers < 1% by SDS-PAGE

Storage buffer

Each vial contains 10 mM Phosphate buffer and 200 mM NaCl (pH7.0) without preservatives.

Storage & Expiration

Ship at 4℃. Upon receipt, store at -20℃ or -80℃ for 2 years. After reconstituting, it can store at 4℃ for a week, it is recommended to add 0.1% HSA or BSA for long term.

Avoid repeated freeze and thaw cycles.

Background

Ciliary neurotrophic factor is a protein that in humans is encoded by the CNTF gene.The protein encoded by this gene is a polypeptide hormone and nerve growth factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations including astrocytes. It was first characterized as a trophic factor for motor neurons in the ciliary ganglion and spinal cord, leading to its evaluation in humans suffering from motor neuron disease. CNTF has also been shown to be expressed by cells on the bone surface, and to reduce the activity of bone forming cells, osteoblasts.

Reference

1. Lambert PD, Anderson KD, Sleeman MW, Wong V, Tan J, Hijarunguru A, Corcoran TL, Murray JD, Thabet KE, Yancopoulos GD, Wiegand SJ (2001). "Ciliary neurotrophic factor activates leptin-like pathways and reduces body fat, without cachexia or rebound weight gain, even in leptin-resistant obesity". Proc. Natl. Acad. Sci. U.S.A. 98 (8): 4652–7.

2. McGregor NE, Poulton IJ, Walker EC, Pompolo S, Quinn JM, Martin TJ, Sims NA (Mar 2010). "Ciliary neurotrophic factor inhibits bone formation and plays a sex-specific role in bone growth and remodeling". Calcified Tissue International 86 (3): 261–70.