Cytokeratin 8, CK8 Protein (Active)

Catalog# BRP1005

Lot # Check on the product label

Size 100 µg

Description

Recombinant full length human Cytokeratin 8 protein with molecular weight 53 kDa.

Synonyms Keratin type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, KRT8, CK8

Source E. Coli

Purity >95% by SDS-PAGE

Formulation Liquid

Concentration 1 mg/ml

Application

ELISA

SDS-PAGE

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage buffer

Each vial contains Tris-HCL (pH7.4 ± 0.2) with 0.02% sodium azide.

Storage & Expiration

Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term.

Avoid repeated freeze and thaw cycles.

Background

Keratin, type II cytoskeletal 8 also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that in human is encoded by the KRT8 gene. CK8 gene contains 8 exons instead of 9 as is found in all other type II cytokeratins due to lack of intron 5, and the gene spans over 8.8 kb. The soluble depolymerized K8 subunits were phosphorylated on ser73 by c-Jun N-terminal kinase upon stimulation of the proapoptotic cytokine receptor Fas in colon carcinoma cells. K8 was also phosphorylated following exposure to ultraviolet light. Keratin 8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.

Reference

1. Krauss, S., Franke, W. W. Organization and sequence of the human gene encoding cytokeratin 8. Gene 86: 241-249, 1990.

2. He, T., Stepulak, A., Holmstrom, T. H., Omary, M. B., Eriksson, J. E. The intermediate filament protein kinase 8 is a novel cytoplasmic substrate for c-Jun N-terminal kinase. J. Biol. Chem. 277: 10767-10774, 2002.

3. W. Jeffrey Allard, Jeri Matera, M. Craig Miller, et al. (October 2004). "Tumor Cells Circulate in the Peripheral Blood of All Major Carcinomas but not in Healthy Subjects or Patients With Nonmalignant Diseases". Clin. Cancer Research 10 (20): 6897–6904.