Cytokeratin 20, CK20 Protein (Active)

Catalog# BRP1008

Lot # Check on the product label

Size 100 µg

Description

Recombinant full length human Cytokeratin 20 protein with molecular weight 46.6 kDa.

Synonyms Keratin type I cytoskeletal 20, Cytokeratin-20, CK-20, Keratin-20, K20, KRT20, CK20

Source E. Coli

Purity >95% by SDS-PAGE

Formulation Liquid

Concentration 0.5 mg/ml

Application

ELISA

SDS-PAGE

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage buffer

Each vial contains Tris-HCL (pH7.4 ± 0.2) with 0.2% sodium azide.

Storage & Expiration

Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term.

Avoid repeated freeze and thaw cycles

Background

Keratin 20, also called Cytokeratin 20, CK20, is a protein that in humans is encoded by the KRT20 gene. KRT20 has an apparent molecular mass of about 46 kD. It is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa. KRT20 formed heterotypic complexes with KRT8 in vitro, and the complexes assembled into intermediate filaments under appropriate conditions. The protein is commonly found in colorectal cancer, transitional cell carcinomas and in Merkel cell tumours, but is absent in lung cancer, prostate cancer, and non-mucinous ovarian cancer. It is often used in combination with antibodies to CK7 to distinguish different types of glandular tumour.

Reference

1. Moll R, Schiller DL, Franke WW (August 1990). "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns". J. Cell Biol. 111 (2): 567–80.

2. Moll, R., Schiller, D. L., Franke, W. W. Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J. Cell Biol. 111: 567-580, 1990.

3. Leong, Anthony S-Y; Cooper, Kumarason; Leong, F Joel W-M (2003). Manual of Diagnostic Cytology (2 ed.). Greenwich Medical Media, Ltd. pp. 171–172.