Epidermal growth factor, EGF Protein (Active)

Catalog# BRP1011

Lot # Check on the product label

Size 1 mg

Description

Recombinant human EGF protein with molecular weight 6.0 kDa.

Synonyms Epidermal growth factor, EGF

Source E. Coli

Purity >95% by HPLC & SDS-PAGE

Formulation Lyophilized

Reconstitution

Reconstitute with sterile 18MΩ-cm H₂O.

Endotoxin level < 0.1 ng/μg (1 EU/μg) by LAL test

Maximal absorption wave 275 ± 3 nm

Dimmers < 1% by SDS-PAGE

Biological Activity 

The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is < 2 ng/ml corresponding to a specific activity of 5 ×10⁵ IU/mg.

Storage buffer

Each vial contains 10 mM Phosphate buffer and 200 mM NaCl (pH7.0) without preservatives.

Storage & Expiration

Ship at 4℃. Upon receipt, store at -20℃ or -80℃ for 2 years. After reconstituting, it can store at 4℃ for a week, it is recommended to add 0.1% HSA or BSA for long term.

Avoid repeated freeze and thaw cycles.

Background

Epidermal growth factor (EGF) is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. It has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF is produced in abundance by the mouse submandibular gland, and it can be found in human platelets, macrophages, urine, saliva, milk, and plasma. It is a magnesiotropic hormone crucial for total body Mg(2+) balance. Futamura et al. (2002) found that chronic treatment of rats with haloperidol had no influence on EGF levels in the brain or serum.

Reference

1. Tsutsumi, O., Kurachi, H., Oka, T. A physiological role of epidermal growth factor in male reproductive function. Science 233: 975-977, 1986.

2. Carpenter, G., Cohen, S. Epidermal growth factor. Ann. Rev. Biochem. 48: 193-216, 1979.

3. Futamura, T., Toyooka, K., Iritani, S., Niizato, K., Nakamura, R., Tsuchiya, K., Someya, T., Kakita, A., Takahashi, H., Nawa, H. Abnormal expression of epidermal growth factor and its receptor in the forebrain and serum of schizophrenic patients. Molec. Psychiat. 7: 673-682, 2002.