Ferritin (L chain) Protein (Active)

Catalog# BRP1016

Lot # Check on the product label

Size 1 mg

Description

Recombinant full length human ferritin (L chain) protein with molecular weight 20 kDa.

Synonyms Ferritin light chain, Ferritin L subunit, FTL

Source E. Coli

Purity >95% by SDS-PAGE

Formulation Liquid

Concentration 1 mg/ml

Application

ELISA

SDS-PAGE

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage buffer

Each vial contains 10 mM Tris-HCL (pH8.8 ± 0.2) with 0.05% sodium azide.

Storage & Expiration

Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term.

Avoid repeated freeze and thaw cycles.

Background

Ferritin is a ubiquitous intracellular protein that stores iron and releases it in a controlled fashion. It is a protein of 450 kDa consisting of 24 subunits that is present in every cell type. In vertebrates, these subunits are both the light (L) and the heavy (H) type with an apparent molecular weight of 19 kDA or 21 kDA respectively; their sequences are about 50% homologous. Ferritin light chain (FTL) is abnormally expressed in fetuses of both IVF and ICSI, which may contribute to the increase risk of birth defects in these ART. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. Mutations of the FTL gene cause the rare adult-onset basal ganglia disease also known as neuroferritinopathy.

Reference

1. Theil, E. (1987). "Ferritin: structure, gene regulation, and cellular function in animals, plants, and microorganisms.". Annual review of biochemistry 56 (1): 289–315.

2. "FTL ferritin, light polypeptide". National Center for Biotechnology Information. 5 July 2009. Retrieved 20 July 2009.

3. Zhang Y, Zhang YL, Feng C, et al. (October 2008). "Comparative proteomic analysis of human placenta derived from assisted reproductive technology". Proteomics 8 (20): 4344–56.