Monoclonal Anti- Tyrosine Hydroxylase, TH Antibody
Size: 100 μg
Host: Mouse
Reactivity: Human, rat, rabbit
Isotype: IgG1
Application: WB, IHC-P, IHC-Fr
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Price: $180.00
Catalog# BMA1044
Lot # Check on the product label
Size 100 µg
Isotype IgG1
Clone # N37
Host Mouse
Reactivity Human, rat, rabbit
Product Form Liquid
Purification Protein A or Protein G purified
Immunogen Purified rat TH.
Recommend Application
Western Blot, WB (1:200-1000)
Immunohistochemistry, IHC-P (1:20-200)
Immunohistochemistry, IHC-Fr (1:20-200)
Other applications have not been tested.
The optimal dilutions should be determined by end user.
Storage Buffer
In TBS (0.1M), 30% Glycerol and 0.01% Sodium Azide.
Storage Instruction
Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).
Avoid repeated freeze and thaw cycles.
Background
Tyrosine hydroxylase or tyrosine 3-monooxygenase is the enzyme responsible for catalyzing the conversion of the amino acid L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA). In humans, tyrosine hydroxylase is encoded by the TH gene, and the enzyme is present in the central nervous system (CNS), peripheral symphatic neurons and the adrenal medulla. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. It also can be inhibited by the drug α-methyl-para-tyrosine (metirosine). This drug is rarely used and can cause depression, but it is useful in treating pheochromocytoma and also resistant hypertension.
Reference
1. Hedstrand H, Ekwall O, Haavik J, Landgren E, Betterle C, Perheentupa J, Gustafsson J, Husebye E, Rorsman F, Kämpe O (2000). "Identification of Tyrosine Hydroxylase as an Autoantigen in Autoimmune Polyendocrine Syndrome Type I". Biochemical and Biophysical Research Communications 267 (1): 456–461.
2. Nagatsu T (1995). "Tyrosine hydroxylase: human isoforms, structure and regulation in physiology and pathology". Essays Biochem. 30: 15–35.
3. Kaufman S (1995). "Tyrosine hydroxylase". Adv. Enzymol. Relat. Areas Mol. Biol.. Advances in Enzymology - and Related Areas of Molecular Biology 70: 103–220.
