Monoclonal Anti- ZFP-42 / REX1 Antibody

Catalog# BMA1046

Lot # Check on the product label

Size 100 μg

Isotype IgG1

Clone # Z4

Host Mouse

Reactivity Human

Product Form Liquid

Purification Protein A purified

Immunogen 

A synthetic peptide (conjugated with KLH) corresponding to the C-terminal of human ZFP-42.

Recommend Application

Western Blot, WB (1:500)

Immunocytochemistry, ICC (1:50)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Zfp-42 (Rex1) is a known marker of pluripotency, and most notably found in undifferentiated embryonic stem cells. It is a 288-amino acid protein and has a calculated molecular mass of 32.3 kD. It has been identified in the testicles, expressed at the spermatocytes in the more inner layers of the testicles. Rex1 has been found to be critically important in maintaining proliferative state in mesenchymal stem cells(MSC), while simultaneously preventing differentiation.

Reference

1. Hosler, B. A., LaRosa, G. J., Grippo, J. F., Gudas, L. J. Expression of REX-1, a gene containing zinc finger motifs, is rapidly reduced by retinoic acid in F9 teratocarcinoma cells. Molec. Cell. Biol. 9: 5623-5629, 1989.