Monoclonal Anti- NT-proBNP (Detection Ab) Antibody

Catalog# BDA1010

Lot # Check on the product label

Size 1 mg

Isotype IgG1

Clone # C7

Host Mouse

Reactivity Human

Product Form Liquid

Purification & Buffer

Protein A or G purified and supplied in 0.01 M PBS (pH7.4) without preservative.

Purity >95% by HPLC & SDS-PAGE

Concentration 6.0 mg/ml

Immunogen 

Recombinant human NT-proBNP.

Specificity It specifically recognize the human NT-proBNP, and does not cross react with BNP and cardiac Troponin I. 

Recommend Application

Western Blot

ELISA

Lateral flow

Chemilumineseent immunoassay, CLIA

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Matched antibody pair

Capture Ab: NT-proBNP pAb (# BDA1009)

Detection Ab: NT-proBNP mAb (clone # C7)

Storage Instruction 

Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

The N-terminal prohormone of brain natriuretic peptide (NT-proBNP) is a 76 amino acid N-terminal fragment of brain natriuretic peptide. Both BNP and NT-proBNP levels in the blood are used for screening, diagnosis of acute congestive heart failure (CHF) and may be useful to establish prognosis in heart failure, as both markers are typically higher in patients with worse outcome.BNP may be a useful screening tool for left ventricular dysfunction in patients with history suggestive of heart disease and be used to assist in forming a pretest probability, which in turn could greatly assist in appropriateness of patient referral and in optimization of drug therapy.

Reference

1. Bhalla V, Willis S, Maisel AS (2004). "B-type natriuretic peptide: the level and the drug--partners in the diagnosis of congestive heart failure". Congest Heart Fail 10 (1 Suppl 1): 3–27.

2. Atisha D, Bhalla MA, Morrison LK, Felicio L, Clopton P, Gardetto N, Kazanegra R, Chiu A, Maisel AS (September 2004). "A prospective study in search of an optimal B-natriuretic peptide level to screen patients for cardiac dysfunction". Am. Heart J. 148 (3): 518–23.