Monoclonal Anti- PCT (Detection Ab) Antibody

Catalog# BDA1014

Lot # Check on the product label

Size 1 mg

Isotype IgG1

Clone # D6

Host Mouse

Reactivity Human

Product Form Liquid

Purification & Buffer

Protein A or G purified and supplied in 0.01 M PBS (pH7.4) & 0.15 M NaCl without preservative.

Purity >95% by HPLC & SDS-PAGE

Immunogen 

Recombinant PCT protein.

Specificity It specifically recognize the human PCT. 

Recommend Application

Western Blot

ELISA

Chemilumineseent immunoassay, CLIA

Immunoturbidimetry, ITM

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Matched antibody pair

Capture Ab: PCT mAb (clone # H6)

Detection Ab: PCT mAb (clone # D6)

Storage Instruction 

Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Procalcitonin (PCT) is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. It is composed of 116 amino acids and is produced by parafollicular cells (C cells) of the thyroid and by the neuroendocrine cells of the lung and the intestine. Measurement of procalcitonin can be used as a marker of severe sepsis caused by bacteria and generally grades well with the degree of sepsis, although levels of procalcitonin in the blood are very low. PCT has the greatest sensitivity (85%) and specificity (91%) for differentiating patients with systemic inflammatory response syndrome (SIRS) from those with sepsis, when compared with IL-2, IL-6, IL-8, CRP and TNF-alpha. It is a biomarker of bacterial infection which may help gauge the severity and prognosis of patients with CAP. In addition to clinical predictors, PCT may assist in decisions pertaining to timing of discharge from hospital and the discontinuation of antibiotics.

Reference

1. Meisner M, Tschaikowsky K, Palmaers T, Schmidt J (1999). "Comparison of procalcitonin (PCT) and C-reactive protein (CRP) plasma concentrations at different SOFA scores during the course of sepsis and MODS". Crit Care 3 (1): 45–50.

2. BalcI C, Sungurtekin H, Gürses E, Sungurtekin U, Kaptanoglu B (February 2003). "Usefulness of procalcitonin for diagnosis of sepsis in the intensive care unit". Crit Care 7 (1): 85–90.

3. Lindstrom S, Wong EK. Procalcitonin, a valuable biomarker assisting clinical decision making in the management of community-acquired pneumonia. Intern Med J. 2014 Feb 14.