Recombinant Human Heparanase, HPSE Protein
Lot # Check on the product label
Size: 50 μg
Description: Partial
Synonyms: HPSE, HPA, HPA1, HPR1, HPSE1, HSE1, heparanase,
Source: E. Coli
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Price: $850.00
Catalog# BRP1129
Lot # Check on the product label
Size 50 μg
Description
Partial
Expression
36-109aa
Heparanase 8 kDa subunit Chain
Sequence
QDVVD LDFFTQEPLH LVSPSFLSVT IDANLATDPR FLILLGSPKL RTLARGLSPA YLRFGGTKTD FLIFDPKKE
Synonyms HPSE, HPA, HPA1, HPR1, HPSE1, HSE1, heparanase
Source E. Coli
Purity >85% by SDS-PAGE
Application
SDS-PAGE
Other applications have not been tested.
The optimal dilutions should be determined by end user.
Storage buffer
Tris-based buffer,50% glycerol
Storage & Expiration
Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term.
Avoid repeated freeze and thaw cycles.
Background
Heparanase (Hpa1 heparanase, Hpa1, heparanase 1, heparanase-1, C1A heparanase, HPSE) is an enzyme with systematic name heparan sulfate N-sulfo-D-glucosamine endoglucanase. heparanase (HPSE) was localized on MTOC in mitotic cells. Induced expression of HPSE in human airway epithelial cells promoted mitotic spindle formation, and silencing of HPSE expression disrupted the mitotic spindle formation, resulting in chromosome instability, including chromosome mis-segregation and increased micronuclei formation. HPSE is an important protein in regulation of mitotic spindle and loss of HPSE function on MTOC associates with chromosome instability, indicating that loss of HPSE on MTOC may play critical roles in oncogenesis.
Reference
1. Yang S, Liao Y, Zhao Q, Xie Y, Zheng A, Wan H. Heparanase Is a Critical Regulator of Mitotic Spindles Required for Maintaining Chromosome Stability. DNA Cell Biol. 2018 Apr;37(4):291-297.