Recombinant Human Heparanase, HPSE Protein

Catalog# BRP1129

Lot # Check on the product label

Size 50 μg

Description

Partial

Expression

36-109aa

Heparanase 8 kDa subunit Chain

Sequence

QDVVD LDFFTQEPLH LVSPSFLSVT IDANLATDPR FLILLGSPKL RTLARGLSPA YLRFGGTKTD FLIFDPKKE

Synonyms HPSE, HPA, HPA1, HPR1, HPSE1, HSE1, heparanase

Source E. Coli

Purity >85% by SDS-PAGE

Application

SDS-PAGE

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage buffer

Tris-based buffer,50% glycerol

Storage & Expiration

Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term.

Avoid repeated freeze and thaw cycles.

Background

Heparanase (Hpa1 heparanase, Hpa1, heparanase 1, heparanase-1, C1A heparanase, HPSE) is an enzyme with systematic name heparan sulfate N-sulfo-D-glucosamine endoglucanase. heparanase (HPSE) was localized on MTOC in mitotic cells. Induced expression of HPSE in human airway epithelial cells promoted mitotic spindle formation, and silencing of HPSE expression disrupted the mitotic spindle formation, resulting in chromosome instability, including chromosome mis-segregation and increased micronuclei formation. HPSE is an important protein in regulation of mitotic spindle and loss of HPSE function on MTOC associates with chromosome instability, indicating that loss of HPSE on MTOC may play critical roles in oncogenesis.

Reference

1. Yang S, Liao Y, Zhao Q, Xie Y, Zheng A, Wan H. Heparanase Is a Critical Regulator of Mitotic Spindles Required for Maintaining Chromosome Stability. DNA Cell Biol. 2018 Apr;37(4):291-297.