Recombinant Vaccinia Virus, VACV A27 Protein

Catalog# BRP1147

Lot # Check on the product label

Size 100 μg

Description

Recombinant VACV A27 protein with his tag.

Source E. Coli

Purity >95% by SDS-PAGE

Purification Affinity purification

Formulation Liquid

Concentration 1 mg/ml

Molecular Weight 13.9kDa

Application

EIA

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage buffer

0.01M PBS, 0.3M Nacl, pH 7.4, 0.1% Proclin300

Storage & Expiration

Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term.

Avoid repeated freeze and thaw cycles.

Background

Vaccinia virus (VACV or VV) is a large, complex, enveloped virus belonging to the poxvirus family. It has a linear, double-stranded DNA genome approximately 190 kbp in length, which encodes approximately 250 genes. The dimensions of the virion are roughly 360 × 270 × 250 nm, with a mass of approximately 5–10 fg. The vaccinia virus is the source of the modern smallpox vaccine, which the World Health Organisation used to eradicate smallpox in a global vaccination campaign in 1958–1977. Although smallpox no longer exists in the wild, vaccinia virus is still studied widely by scientists as a tool for gene therapy and genetic engineering.

Reference

1. Ryan KJ, Ray CG, eds. (2004). Sherris Medical Microbiology (4th ed.). McGraw Hill.

2. Johnson, L.; Gupta, A. K.; Ghafoor, A.; Akin, D.; Bashir, R. (2006). "Characterization of vaccinia virus particles using microscale silicon cantilever resonators and atomic force microscopy". Sensors and Actuators B Chemical. 115 (1): 189–197.