Chloramphenicol, CAP ELISA Kit

Catalog No.: BCEK1001

Size: 96T

Range: 0.1 ppb- 8.1 ppb

Sensitivity: 0.1 ppb (µg / kg)

Detection time: 50 min

Storage and Expiration: Store at 2-8℃ for one year. 

Application: For quantitative detection of Chloramphenicol in animal tissues, honey and feed samples.

Principle of the Assay  

This kit was based on an indirect competitive enzyme immunoassay. Its main principle is that the unlabeled antibody is incubated in the presence of its antigen (sample). These bound antibody/antigen complexes are then added to an antigen-coated well. The plate is washed, so unbound antibody is removed. (The more antigen in the sample, the more Ag-Ab complexes are formed and so there are less unbound antibodies available to bind to the antigen in the well, hence "competition".) The secondary antibody, specific to the primary antibody, is added. This second antibody is coupled to the enzyme. A substrate is added, and remaining enzymes elicit a chromogenic or fluorescent signal. The reaction is stopped to prevent eventual saturation of the signal.

Kit components

1. One 96-well Chloramphenicol microtiter plate (12 strips x 8 wells)

2. Standards: 0 ppb (blank / negative control), 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb (RTU), 1ml for each

3. Chloramphenicol standard with high concentration (100 ppb): 0.5 ml

4. Chloramphenicol HRP conjugated reagent: 13 ml

5. Anti- Chloramphenicol antibody: 7 ml

6. Sample diluent buffer (2 x): 40 ml

7. Substrate A: 7 ml

8. Substrate B: 7 ml

9. Stop solution:  7 ml

10. Wash buffer (20 x):  40 ml

Material Required But Not Provided

1. Microtiter plate spectrophotometer (450 / 630 nm)

2. Homogenizer, centrifuge & centrifuge tubes (5 ml, 50ml)

3. Precise pipette and disposable pipette tips

4. ELISA shaker

5. Ethyl acetate, n-Hexane

6. Deionized water