Nitrofurantoin, AHD ELISA Kit

Catalog No.: BCEK1004

Size: 96T

Range: 0.1 ppb- 8.1 ppb

Sensitivity: 0.1 ppb (µg / kg)

Detection time: 70 min

Storage and Expiration: Store at 2-8℃ for one year. 

Application: For quantitative detection of Nitrofurantoin in animal tissues and honey samples.

Principle of the Assay  

This kit was based on an indirect competitive enzyme immunoassay. Its main principle is that the unlabeled antibody is incubated in the presence of its antigen (sample). These bound antibody/antigen complexes are then added to an antigen-coated well. The plate is washed, so unbound antibody is removed. (The more antigen in the sample, the more Ag-Ab complexes are formed and so there are less unbound antibodies available to bind to the antigen in the well, hence "competition".) The secondary antibody, specific to the primary antibody, is added. This second antibody is coupled to the enzyme. A substrate is added, and remaining enzymes elicit a chromogenic or fluorescent signal. The reaction is stopped to prevent eventual saturation of the signal.

Kit components

1. One 96-well Nitrofurantoin microtiter plate (12 strips x 8 wells)

2. Standards: 0 ppb (blank / negative control), 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb (RTU), 1ml for each

3. Nitrofurantoin HRP conjugated reagent: 13 ml

4. Anti- Nitrofurantoin antibody: 7 ml

5. Honey sample diluent buffer (2 x): 45 ml

6. Tissues sample diluent buffer (2 x): 45 ml

7. Substrate A: 13 ml

8. Substrate B: 13 ml

9. Stop solution:  13 ml

10. Wash buffer (20 x): 40 ml

11. Nitrofurantoin derivatization reagent: 10 ml

Material Required But Not Provided

1. Microtiter plate spectrophotometer (450 / 630 nm)

2. Homogenizer, centrifuge & centrifuge tubes (5 ml, 50ml)

3. Precise pipette and disposable pipette tips

4. ELISA shaker

5. Ethyl acetate, concentrated hydrochloric acid, sodium hydroxide

6. Deionized water