Oxytetracycline ELISA Kit (Honey)

Catalog No.: BCEK1011

Size: 96T

Range: 5 ppb- 405 ppb

Sensitivity: 0.1 ppb (µg / kg)

Detection time: 45 min

Storage and Expiration: Store at 2-8℃ for one year. 

Application: For quantitative detection of Oxytetracycline in honey samples.

Principle of the Assay  

This kit was based on an indirect competitive enzyme immunoassay. Its main principle is that the unlabeled antibody is incubated in the presence of its antigen (sample). These bound antibody/antigen complexes are then added to an antigen-coated well. The plate is washed, so unbound antibody is removed. (The more antigen in the sample, the more Ag-Ab complexes are formed and so there are less unbound antibodies available to bind to the antigen in the well, hence "competition".) The secondary antibody, specific to the primary antibody, is added. This second antibody is coupled to the enzyme. A substrate is added, and remaining enzymes elicit a chromogenic or fluorescent signal. The reaction is stopped to prevent eventual saturation of the signal.

Kit components

1. One 96-well Oxytetracycline microtiter plate (12 strips x 8 wells)

2. Standards (50×): 0 ppb (blank / negative control), 0 ppb, 5 ppb,15 ppb, 45ppb, 135ppb, 405 ppb (RTU), 1ml for each

3. Standards(High concentration) 1ppm: 0.5 ml

4. Oxytetracycline HRP conjugated reagent: 7 ml

5. Anti- Oxytetracycline antibody: 7 ml

6. Oxytetracycline standard diluent buffer: 30 ml

7. Substrate A: 7 ml

8. Substrate B: 7 ml

9. Stop solution:  7 ml

10. Wash buffer (20 x): 40 ml

Material Required But Not Provided

1. Microtiter plate spectrophotometer (450 / 630 nm)

2. Homogenizer, centrifuge & centrifuge tubes (5 ml, 50ml)

3. Precise pipette and disposable pipette tips

4. ELISA shaker

5. Ethyl acetate, concentrated hydrochloric acid, sodium hydroxide

6. Deionized water