Prestained Multicolor Protein Molecular Weight Marker (10-250 kDa)

Catalog# BWR1074  

Size: 100 μl (for 10-20 times)

Lot # Check on the product label

Storage: Store at 4°C for 3 months, and at -20°C for long term.

Storage Buffer

62.5 mM Tris-H₃PO₄ (pH 7.5, 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃ and 33% glycerol.  

Features

• Molecular weight range – 10-250 kDa.
• Brightness reference band-70 kDa, 25 kDa.
• Ready to use-Pre-mixed with loading buffer.
• Narrow band.

Application

• Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis. 
• Verifying Western transfer efficiency.
• Approximate sizing of proteins on SDS-PAGE and Western blots.

Introductions

Prestained protein ladder / marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verifying of Western transfer efficiency on PVDF, nylon and nitrocellulose membranes and approximate sizing of proteins. The protein ladder is composed of 9 prestained recombinant proteins with high-purity. These proteins are stained with 3 different dyes with molecular weights from 10 kDa to 250 kDa. It has two orange reference bands–70 kDa band/filedownload/806543 and 25 kDa band.

Protocol

1. Thaw the ladder either at room temperature or at 37°C for a few minutes to dissolve precipitated solids thoroughly. Do not boil!

2. Mix gently, but thoroughly, to ensure that the solution is homogeneous.  

3. Load the following volumes of the ladder on

    Min SDS-polyacrylamide gel: 5 μl per well, with a thickness of 0.75-1.0 mm

   Large SDS-polyacrylamide gel: 10 μl per well, with a thickness of 0.75-1.0 mm